TY - JOUR
T1 - An experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated piglets
AU - Martínez-Olondris, P.
AU - Sibila, O.
AU - Agustí, C.
AU - Rigol, M.
AU - Soy, D.
AU - Esquinas, C.
AU - Piñer, R.
AU - Luque, N.
AU - Guerrero, L.
AU - Quera, M. Á
AU - Marco, F.
AU - De La Bellacasa, J. P.
AU - Ramirez, J.
AU - Torres, A.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - The objectives of the study were to validate a model of methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in ventilated piglets and to study the time-course of biological markers and histopathological changes. 12 piglets were intubated and inoculated with 15 mL of a suspension of 106 colony forming units of MRSA in every lobe through the bronchoscope channel. The piglets were ventilated for 12 h (n=6) and 24 h (n=6). Clinical parameters were assessed every 6 h and pro-inflammatory cytokines were measured in serum and in bronchoalveolar lavage (BAL) at baseline and sacrifice. Histopathology of each lobe and cultures from blood, lungs and BAL were performed. Animals developed histopathological evidence of pneumonia at necropsy. At 12 h, pneumonia was present in all animals and was severe pneumonia at 24 h. Microbiological studies confirmed the presence of MRSA. A significant increase in interleukin (IL)-6, IL-8 and tumour necrosis factor-α values was seen in BAL at 24 h and IL-6 at 12 h. In serum, only IL-6 levels had increased significantly at 24 h. In ventilated piglets, bronchoscopic inoculation of MRSA induces pneumonia at 12 h and severe pneumonia at 24 h. This severity was associated with a corresponding increase in systemic and local inflammatory response. Copyright
AB - The objectives of the study were to validate a model of methicillin-resistant Staphylococcus aureus (MRSA) pneumonia in ventilated piglets and to study the time-course of biological markers and histopathological changes. 12 piglets were intubated and inoculated with 15 mL of a suspension of 106 colony forming units of MRSA in every lobe through the bronchoscope channel. The piglets were ventilated for 12 h (n=6) and 24 h (n=6). Clinical parameters were assessed every 6 h and pro-inflammatory cytokines were measured in serum and in bronchoalveolar lavage (BAL) at baseline and sacrifice. Histopathology of each lobe and cultures from blood, lungs and BAL were performed. Animals developed histopathological evidence of pneumonia at necropsy. At 12 h, pneumonia was present in all animals and was severe pneumonia at 24 h. Microbiological studies confirmed the presence of MRSA. A significant increase in interleukin (IL)-6, IL-8 and tumour necrosis factor-α values was seen in BAL at 24 h and IL-6 at 12 h. In serum, only IL-6 levels had increased significantly at 24 h. In ventilated piglets, bronchoscopic inoculation of MRSA induces pneumonia at 12 h and severe pneumonia at 24 h. This severity was associated with a corresponding increase in systemic and local inflammatory response. Copyright
KW - Animal model
KW - Inflammatory response
KW - Methicillin-resistant Staphylococcus aureus
KW - Ventilator-associated pneumonia
UR - http://www.scopus.com/inward/record.url?scp=77957842902&partnerID=8YFLogxK
U2 - 10.1183/09031936.00176709
DO - 10.1183/09031936.00176709
M3 - Article
C2 - 20351024
AN - SCOPUS:77957842902
SN - 0903-1936
VL - 36
SP - 901
EP - 906
JO - European Respiratory Journal
JF - European Respiratory Journal
IS - 4
ER -