TY - JOUR
T1 - Anaerobic digestion of aqueous phase from hydrothermal liquefaction of Spirulina using biostimulated sludge
AU - Egerland Bueno, Beatriz
AU - Américo Soares, Laís
AU - Quispe-Arpasi, Diana
AU - Kimiko Sakamoto, Isabel
AU - Zhang, Yuanhui
AU - Amancio Varesche, Maria Bernadete
AU - Ribeiro, Rogers
AU - Tommaso, Giovana
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/9
Y1 - 2020/9
N2 - Hydrothermal liquefaction is a process that converts wet biomass into biofuels, more specifically bio-crude oil. During the process, post hydrothermal liquefaction waste water (PHWW) is generated, rich in nutrient and organic matter, however potentially toxic. Anaerobic digestion of PHWW from Spirulina, was evaluated using biostimulated sludge as a strategy to optimize the process. The biostimulation was conducted in a sequential batch reactor fed with organic acids and methanol aiming at development of acetogenic and methanogenic microorganism. Anaerobic biodegradability batch assays were performed, with biostimulated sludge and with non-biostimulated sludge, using increasing PHWW concentrations. Biostimulated sludge were more favourable for reaching higher methane yields at higher organic matter concentrations in comparison to non-biostimulated sludge, presenting less inhibition at conditions tested. Biostimulation was a key process to select and favour potential microorganisms involved in specialized uptake of recalcitrant compounds, such as Mesotoga and Methanomethylovorans.
AB - Hydrothermal liquefaction is a process that converts wet biomass into biofuels, more specifically bio-crude oil. During the process, post hydrothermal liquefaction waste water (PHWW) is generated, rich in nutrient and organic matter, however potentially toxic. Anaerobic digestion of PHWW from Spirulina, was evaluated using biostimulated sludge as a strategy to optimize the process. The biostimulation was conducted in a sequential batch reactor fed with organic acids and methanol aiming at development of acetogenic and methanogenic microorganism. Anaerobic biodegradability batch assays were performed, with biostimulated sludge and with non-biostimulated sludge, using increasing PHWW concentrations. Biostimulated sludge were more favourable for reaching higher methane yields at higher organic matter concentrations in comparison to non-biostimulated sludge, presenting less inhibition at conditions tested. Biostimulation was a key process to select and favour potential microorganisms involved in specialized uptake of recalcitrant compounds, such as Mesotoga and Methanomethylovorans.
KW - 16S rDNA sequencing
KW - Biodegradation
KW - PCR/DGGE
KW - Taxonomical potential
KW - Toxic wastewaters
UR - http://www.scopus.com/inward/record.url?scp=85085700699&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2020.123552
DO - 10.1016/j.biortech.2020.123552
M3 - Article
C2 - 32502889
AN - SCOPUS:85085700699
SN - 0960-8524
VL - 312
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 123552
ER -