TY - JOUR
T1 - GENES RESISTENTES A CARBAPENÉMICOS Y COLISTINA AISLADOS EN Musca domestica PROVENIENTE DE UN BASUR AL CERCANO A UN HOSPITAL DE LIMA
AU - Alarcón-Calle, Miguel A.
AU - Osorio-Guevara, Víctor L.
AU - Salas-Asencios, Ramsés
AU - Yareta, José
AU - Marcos-Carbajal, Pool
AU - Rodrigo-Rojas, María E.
N1 - Publisher Copyright:
© 2024, Revista Peruana de Medicina Experimental y Salud Pública.
PY - 2024/6
Y1 - 2024/6
N2 - The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicro-bial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1.
AB - The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicro-bial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1.
KW - Carbapenem
KW - Colistin
KW - Drug Resistance
KW - Houseflies
UR - http://www.scopus.com/inward/record.url?scp=85202006971&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2024.412.13257
DO - 10.17843/rpmesp.2024.412.13257
M3 - Artículo
C2 - 39166639
AN - SCOPUS:85202006971
SN - 1726-4634
VL - 41
SP - 164
EP - 170
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 2
ER -