TY - JOUR
T1 - Hospital effluents as a reservoir of beta-lactamase-and carbapenemase-producing enterobacteriaceae
AU - Soriano-Moreno, David R.
AU - Yareta, José
AU - Rojas-Cosi, Ana F.
AU - Fajardo-Loyola, Alexander
AU - León-Luna, Diana
AU - Castillo-Quezada, Isabel
AU - Laura-Bejarano, Mario
AU - Hilario-Sánchez, Milagros
AU - Galarza-Pérez, Marco
AU - Marcos-Carbajal, Pool
N1 - Publisher Copyright:
© 2021, Instituto Nacional de Salud. All rights reserved.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - The aim of this study was to determine the presence of beta-lactamase-(bla) producing Enterobacteriaceae in hospital effluent samples from two level II and III hospitals in Lima, Peru. The resistance profile of the isolated bacteria was identified and characterized using the MicroScan system for 18 antimicrobials, and the presence of extended spectrum beta-lactamases (ESBL) (blaCTX-M, blaSHV blaTEM, blaPER ) and carbapenemases (blaKPC, blaNDM, blaVIM, blaIMP ) resistance genes was determined by conventional PCR. Thirty-two isolates were identified (20 Enterobacteriaceae and 12 gram-negative bacteria). All the isolated bacteria showed multidrug resistance. ESBL (blaTEM ) and carbapenemase (blaKPC, blaIMP) genes were found in samples from the hospitals that we evaluated. The release of these microorganisms to public areas and the lack of treatment of the hospital effluents could be an important public health problem.
AB - The aim of this study was to determine the presence of beta-lactamase-(bla) producing Enterobacteriaceae in hospital effluent samples from two level II and III hospitals in Lima, Peru. The resistance profile of the isolated bacteria was identified and characterized using the MicroScan system for 18 antimicrobials, and the presence of extended spectrum beta-lactamases (ESBL) (blaCTX-M, blaSHV blaTEM, blaPER ) and carbapenemases (blaKPC, blaNDM, blaVIM, blaIMP ) resistance genes was determined by conventional PCR. Thirty-two isolates were identified (20 Enterobacteriaceae and 12 gram-negative bacteria). All the isolated bacteria showed multidrug resistance. ESBL (blaTEM ) and carbapenemase (blaKPC, blaIMP) genes were found in samples from the hospitals that we evaluated. The release of these microorganisms to public areas and the lack of treatment of the hospital effluents could be an important public health problem.
KW - Antibacterial agents
KW - Antibacterial drug resistance
KW - Multiple antibacterial drug resistance
KW - Peru (Source: MeSH NLM)
KW - Public health
KW - Public hospitals
KW - Sewerage
KW - Waste water
UR - http://www.scopus.com/inward/record.url?scp=85113969754&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2021.382.6202
DO - 10.17843/rpmesp.2021.382.6202
M3 - Article
C2 - 34468580
AN - SCOPUS:85113969754
SN - 1726-4634
VL - 38
SP - 302
EP - 307
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 2
ER -