TY - JOUR
T1 - Molecular characterization of multi-resistant enterobacteria in two departments of the Peruvian jungle
AU - León-Luna, Diana
AU - Fajardo-Loyola, Alexander
AU - Yareta-Yareta, José
AU - Burgos-Espejo, Antonio
AU - Peralta-Siesquen, Carlos
AU - Galarza-Pérez, Marco
AU - Marcos-Carbajal, Pool
N1 - Publisher Copyright:
© 2021. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Introduction: The emergence of multi-resistant enterobacteria and extended spectrum betalactamase producers in external consultation patients with urinary tract infections represents a public health problem in Peru. Objectives. Molecularly characterize multi-resistant enterobacteria isolated from patients diagnosed with urinary tract infection from two departments of the Peruvian jungle. Materials and methods. A descriptive, observational and retrospective study o 61 uroculture isolates from the Peruvian jungle was conducted during 2017 -2018. Resistance profiles were identified using the MicroScan automated system®. A conventional polymerase chain reaction was used for the detection of the genes blacTx-M, blaTEM, blasHv. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli, Madre de Dios 25% (10/40) and Ucayali 76.2% (16/21). For both departments the gene blacrx-M was the most prevalent with 41 % (25/61), followed by blaTEM with 24.6% (15/61) and blasHv with 16.4% (10/61). Antimicrobial susceptibility profile detected resistance levels with 72.6% for ampicillin, 82.3% cephalotin and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4%; with the blacrx-M gene being the most common.
AB - Introduction: The emergence of multi-resistant enterobacteria and extended spectrum betalactamase producers in external consultation patients with urinary tract infections represents a public health problem in Peru. Objectives. Molecularly characterize multi-resistant enterobacteria isolated from patients diagnosed with urinary tract infection from two departments of the Peruvian jungle. Materials and methods. A descriptive, observational and retrospective study o 61 uroculture isolates from the Peruvian jungle was conducted during 2017 -2018. Resistance profiles were identified using the MicroScan automated system®. A conventional polymerase chain reaction was used for the detection of the genes blacTx-M, blaTEM, blasHv. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli, Madre de Dios 25% (10/40) and Ucayali 76.2% (16/21). For both departments the gene blacrx-M was the most prevalent with 41 % (25/61), followed by blaTEM with 24.6% (15/61) and blasHv with 16.4% (10/61). Antimicrobial susceptibility profile detected resistance levels with 72.6% for ampicillin, 82.3% cephalotin and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4%; with the blacrx-M gene being the most common.
KW - Enterobacteriaceae
KW - beta-Lactam resistance
KW - drug resistance
KW - farmacorresistencia microbiana
KW - genes
KW - microbial
KW - resistencia betalactámica
UR - http://www.scopus.com/inward/record.url?scp=85115766068&partnerID=8YFLogxK
U2 - 10.7705/biomedica.5720
DO - 10.7705/biomedica.5720
M3 - Article
C2 - 34669288
AN - SCOPUS:85115766068
SN - 0120-4157
VL - 41
SP - 1
EP - 22
JO - Biomedica
JF - Biomedica
ER -