TY - JOUR
T1 - DETECCIÓN DE VARIANTES DEL SARS-CoV-2 EN AGUAS RESIDUALES DE HOSPITALES EN PERÚ, 2022
AU - Marcos-Carbajal, Pool
AU - Yareta-Yareta, José
AU - Otiniano-Trujillo, Miguel
AU - Galarza-Pérez, Marco
AU - Espinoza-Culupu, Abraham
AU - Ramirez-Melgar, Jorge L.
AU - Chambi-Quispe, Mario
AU - Luque-Chipana, Néstor Alejandro
AU - Ajalcriña, Rosmery Gutiérrez
AU - Cruz, Victor Sucñer
AU - Chegne, Segundo Nicolas López
AU - Ruiz, Diana Santillán
AU - Chavez, Luis Felipe Segura
AU - Garay, Cinthia Esther Sias
AU - Granara, Alberto Salazar
AU - Cisneros, Pablo Tsukayama
AU - Paniagua, Silvana Teresa Tapia
AU - González-Domenech, Carmen María
N1 - Publisher Copyright:
© 2024, Revista Peruana de Medicina Experimental y Salud Públic.
PY - 2024/4/1
Y1 - 2024/4/1
N2 - Objetive. To identify the presence of the SARS-CoV-2 virus in wastewater from hospitals in Peru. Materials and methods. Water samples were collected from the effluents of nine hospitals in Peru during March and September 2022. SARS-CoV-2 was identified by using Illumina sequencing. Variant, lineage and clade assignments were carried out using the Illumina and Nextclado tools. We verified whether the SARS-CoV-2 variants obtained from wastewater were similar to those reported by the National Institute of Health of Peru from patients during the same period and region. Results. Eighteen of the 20 hospital wastewater samples (90%) provided sequences of sufficient quality to be classified as the Omicron variant according to the WHO classification. Among them, six (30%) were assigned by Nextclade to clades 21K lineage BA.1.1 (n=1), 21L lineage BA.2 (n=2), and 22B lineages BA.5.1 (n=2) and BA.5.5 (n=1). Conclusions. SARS-CoV-2 variants were found in hospital wastewater samples and were similar to those reported by the surveillance system in patients during the same weeks and geographic areas. Wastewater monitoring could provide information on the environmental and temporal variation of viruses such as SARS-CoV-2.
AB - Objetive. To identify the presence of the SARS-CoV-2 virus in wastewater from hospitals in Peru. Materials and methods. Water samples were collected from the effluents of nine hospitals in Peru during March and September 2022. SARS-CoV-2 was identified by using Illumina sequencing. Variant, lineage and clade assignments were carried out using the Illumina and Nextclado tools. We verified whether the SARS-CoV-2 variants obtained from wastewater were similar to those reported by the National Institute of Health of Peru from patients during the same period and region. Results. Eighteen of the 20 hospital wastewater samples (90%) provided sequences of sufficient quality to be classified as the Omicron variant according to the WHO classification. Among them, six (30%) were assigned by Nextclade to clades 21K lineage BA.1.1 (n=1), 21L lineage BA.2 (n=2), and 22B lineages BA.5.1 (n=2) and BA.5.5 (n=1). Conclusions. SARS-CoV-2 variants were found in hospital wastewater samples and were similar to those reported by the surveillance system in patients during the same weeks and geographic areas. Wastewater monitoring could provide information on the environmental and temporal variation of viruses such as SARS-CoV-2.
KW - Genomics, SARS-CoV-2
KW - Sequencing
KW - Wastewater
KW - Wastewater-Based Epidemiological Monitoring
UR - http://www.scopus.com/inward/record.url?scp=85202002138&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2024.412.13484
DO - 10.17843/rpmesp.2024.412.13484
M3 - Artículo
C2 - 39166636
AN - SCOPUS:85202002138
SN - 1726-4634
VL - 41
SP - 140
EP - 145
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 2
ER -
