TY - JOUR
T1 - Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis
AU - Asencios, Luis
AU - Galarza, Marco
AU - Quispe, Neyda
AU - Vásquez, Lucy
AU - Leo, Elena
AU - Valencia, Eddy
AU - Ramírez, Juan
AU - Acurio, Margoth
AU - Salazar, Rosario
AU - Mendoza-Ticona, Alberto
AU - Cáceres, Omar
PY - 2012/3
Y1 - 2012/3
N2 - The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.
AB - The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.
KW - Isoniazid
KW - Molecular Diagnostic Techniques
KW - Multidrug-Resistant
KW - Mutation
KW - Peru
KW - Rifampin
KW - Tuberculosis
KW - Validity of Tests
UR - http://www.scopus.com/inward/record.url?scp=84859992633&partnerID=8YFLogxK
U2 - 10.1590/S1726-46342012000100014
DO - 10.1590/S1726-46342012000100014
M3 - Article
AN - SCOPUS:84859992633
SN - 1726-4634
VL - 29
SP - 92
EP - 98
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 1
ER -