Producción Científica UPeU

URI permanente para esta comunidadhttps://cris.upeu.edu.pe/handle/123456789/1

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    Development and validation of versatile species-specific primer assays for eDNA monitoring and authentication of 10 commercially important Peruvian marine species
    (2025-07-02)
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    R. Alfaro
    ;
    Lorenzo E. Reyes‐Flores
    ;
    Claudia Ingar
    ;
    Luis E. Santos‐Rojas
    Molecular identification assays provide crucial support in the research and regulation of aquatic resources. Among them, species-specific primers provide significant discriminatory power for fast and simultaneous differentiation of closely related species. In this study, we developed species-specific primers for environmental DNA (eDNA) monitoring and identification of 10 fish and shellfish species commonly found in the Peruvian seafood sector. To ensure versatility and high specificity, our primers were subjected to various testing methods including PCR, qPCR, and DNA sequencing, supported by robust validation assays. This validation process included a) an in-silico stage using self-generated and public DNA sequences; b) an in-vitro stage using target species sourced from vouchered specimens, as well as fresh and cooked commercial samples, early life stages, and a wide range of non-target species; and c) an in-situ stage using eDNA samples collected from different Peruvian marine ecosystems. Our novel species-specific primers successfully passed the validation process, demonstrating high efficiency and specificity by unequivocally identifying all target species with 100% accuracy and without cross-species reactions. These primers are thus valuable tools for eDNA monitoring, seafood authentication, and combating illegal, unreported, and unregulated fishing. The assays presented in this study can support effective fishery management and conservation efforts not only in the Peruvian fishery sector but also in other countries where our target species are present or imported.
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    Cross-cultural measurement invariance of the purpose in life test - Short form (PIL-SF) in seven Latin American countries
    (2022-07-28)
    Tomás Caycho‐Rodríguez
    ;
    Lindsey W. Vilca
    ;
    Mauricio Cervigni
    ;
    Miguel Gallegos
    ;
    Pablo Martino
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    Sample size in quantitative instrument validation studies: A systematic review of articles published in Scopus, 2021
    (2022-12-01)
    BACKGROUND: Due to the range of conflicting criteria regarding minimum sample size needed for a scale/questionnaire validation study, the objective of this review is to analyze sample sizes used in published journal articles to contribute a pragmatic perspective to the discussion on sample sizes. METHODS: A sample of 1999 articles published in a Scopus-indexed journal about the validation of a scale or questionnaire during 2021 were analyzed for this study. Abstracts from these articles were tabulated by two data entry professionals and any discrepancies were reviewed by the author. The sample size data was grouped by highest quartile of the journal publishing the article and further sub-categorized based on the inclusion of medical patients or students in each study's population. RESULTS: From the total sample, 1750 articles provided sufficient information in their summary to determine the sample size used. Of these, the majority were published in quartile 1 (784) and quartile 2 (620) journals. Mean values by quartile ranged from 389 (quartile 3) to 2032 (quartile 1), but extreme outliers limited the usefulness of the simple mean. Thus, outlier-removed means were calculated, and in most cases, these sample size values were higher for studies involving students and lower for studies involving patients. DISCUSSION: This study is limited by its focus on a single database and by including all phases of validation from initial quantitative instrument design studies (which tend to have the lowest sample sizes) up to international macro-studies (which can have hundreds of thousands of participants.) Nevertheless, the results of this study provide an additional practical perspective for the academic discussion regarding minimum sample size based on accepted practice.
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    MEASURING BOREDOM DURING THE COVID-19 PANDEMIC
    (2023-04-30)
    José Ventura‐León
    ;
    Tomás Caycho‐Rodríguez
    ;
    Brian Norman Peña-Calero
    ;
    The aim of this study was to translate into Spanish and validate the Boredom Proneness Scale-Short (SBPS) in a sample of young people and adults in Peru during the COVID-19 pandemic. A total of 588 people between 17 and 53 years of age (M = 21.70; SD = 5.22) was selected as a sample. The methodology used combined Confirmatory Factor Analysis (CFA) and Item Response Theory (IRT) to determine the internal structure, factor weights and reliability, which was estimated by means of the omega coefficient test information functioning and empirical or marginal reliability. Evidence of convergent validity of the SBPS was explored based on its relationship with the Generalized Anxiety Disorder Scale (GAD-2) and Patient Health Questionnaire (PHQ-2). Results reveal that the SBPS is a measure that should be interpreted uni-dimensionally. Reliability was excellent and convergence with the GAD-2 and PHQ-2 presented the expected relationship in both magnitude and direction.
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    Academic co-creation: development and validation of a short scale
    (2023-09-21)
    José Ventura‐León
    ;
    Andy Rick Sánchez‐Villena
    ;
    Tomás Caycho‐Rodríguez
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    Introduction Given the profound changes caused in higher education by the COVID-19 pandemic, affecting 1.6 billion students and 63 million educators globally, there arises the necessity for quantifiable measures that capture the essence of academic co-creation. This study aimed to develop and validate a short scale that measures academic co-creation (AC-S) in a sample of higher education students. Methods A total of 3,169 students from three Peruvian cities participated in the study (Mean Age = 25.77 years old; SD = 8.92 years); 1889 were female (59.60%) and 1,280 (40.40%) males. Qualitative and quantitative procedures were used for test construction. Item response theory (IRT) under the two-parameter graded response model (GRM-2PL) and test information function were used to examine reliability; additionally, a brief measure of academic satisfaction was used to provide evidence of relationship with another variable. Results The AC-S displayed strong fit and reliability, assessed through the test information function and standard error. It also showed a moderate correlation with academic satisfaction, bolstering its validity by linking with a pertinent variable. Its brevity enhances its practicality for education and research, efficiently fitting explanatory models and educational contexts. Despite substantial sample size and advanced psychometric methods, the study acknowledges limitations in sample representativeness and cross-sectional design. In conclusion, IRT and SEM techniques compellingly support the AC-S’s reliability and validity. Conclusion The scale’s one-dimensionality, local independence, reliability, and academic satisfaction relationship form a foundation for future exploration of co-creation-based educational models. Further studies should evaluate its performance across diverse cultural contexts.
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    Urea Cycle Enzymatic Abnormalities in Patients in Endogenous Hepatic Coma
    (1963-08-01)
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    María Eugenia Pino
    ;
    Jorge E. Valenzuela
    ;
    F Lorca
  • Publicacion
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    [EFFECTS OF TRIDIHEXETYLCLYMEPHENOXALONE ON INDUCED GASTRIC SECRETION WITH THE MAXIMUM HISTAMINE TEST].
    (1964-10-01)
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    H Iturriaga
    ;
    Jorge E. Valenzuela
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    I Inzunza
    ;
    T Cornejo