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Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
Author(s)
National University of Saint Anthony the Abbot in Cuzco
Antonio Pereira‐Vega
Hospital Juan Ramón Jiménez
Belén Callejón‐Leblic
Universidad de Huelva
Isabel Díaz-Olivares
Instituto de Salud Carlos III
Rafael Santos Santana
Instituto de Salud Carlos III
Carolina Gotera Rivera
Instituto de Salud Carlos III
José Luis Gómez‐Ariza
Universidad de Huelva
José Luís López-Campos
Instituto de Salud Carlos III
Ana Isabel Blanco-Orozco
Instituto de Biomedicina de Sevilla
Luis Seijó
Clinica Universidad de Navarra
María Teresa Nava Rodríguez
Clinica Universidad de Navarra
Luis Alejandro Padrón Fraysse
Hospital Juan Ramón Jiménez
Ángeles Herrera-Chilla
Hospital Juan Ramón Jiménez
Germán Peces‐Barba
Instituto de Salud Carlos III
Tamara García‐Barrera
Universidad de Huelva
Date Issued
16 de octubre de 2023
Type
Article
Volume
22
Issue
11
Series/Report No.
Journal of Proteome Research
Start Page
3499
End Page
3507
ISSN
1535-3893
1535-3907
Abstract
Lung cancer (LC) is a leading cause of mortality, claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in its early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to assess the response to treatment or the detection of cancer recurrence. In this study, we have evaluated the metabolomic profile of serum samples (n = 110) from preoperative (PRE) and postoperative (POST) LC patients collected at two different time points (1 month, A; 3–6 months, B) with respect to healthy people. An untargeted metabolomic platform based on reversed phase (RP) and hydrophilic interaction chromatography (HILIC), using ultra-high performance liquid chromatography (UHPLC) and mass spectrometry (MS), was applied (MassIVE ID MSV000092213). Twenty-two altered metabolites were annotated by comparing all the different studied groups. DG(14,0/22:1), stearamide, proline, and E,e-carotene-3,3′-dione were found altered in PRE, and their levels returned to those of a baseline control group 3–6 months after surgery. Furthermore, 3-galactosyllactose levels remained altered after intervention in some patients. This study provides unique insights into the metabolic profiles of LC patients after surgery at two different time points by combining complementary analytical methods.
Keywords