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Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race
Author(s)
Unchupaico-Payano, Ide
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Alponte-Sierra, Alberto
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Quispe, Carlos
Los Andes Peruvian University
Ancco, Edith
Los Andes Peruvian University
Mariños-Alfaro, Julio César
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Patiño-Rivera, Alberto Rivelino
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Lavado-Meza, Carmencita R.
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Huanca-Rojas, Lupe
Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa
Bazán-Alonso, Luis
Universidad Nacional del Centro del Perú
Date Issued
28 de septiembre de 2022
Type
Article
Publisher
Universidad del Zulia, Facultad de Ciencias Veterinarias
Volume
32
Series/Report No.
Revista Científica de la Facultad de Ciencias Veterinarias
Contained in
Revista Cientifica de la Facultad de Veterinaria
ISSN
0798-2259
Abstract
The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.