Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente
Author(s)
Marco Galarza
Silvia Capristano
Oscar Pellón-Cárdenas
César Sánchez
Jorge Ballon
Heinner Guio
Date Issued
24 de marzo de 2020
Type
Article
Volume
37
Issue
1
Start Page
51
End Page
6
Abstract
OBJECTIVES: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. MATE RIALS AND METHODS: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). RESULTS: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). CONCLUSION: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.
Subjects