Cargando...
Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente
Author(s)
Universidad Nacional de San Agustin de Arequipa
Marco Galarza
Instituto Nacional de Salud
Silvia Capristano
Instituto Nacional de Salud
Oscar Pellón-Cárdenas
Instituto Nacional de Salud
César Sánchez
Instituto Nacional de Salud
Jorge Ballon
Universidad Nacional de San Agustin de Arequipa
Heinner Guio
Instituto Nacional de Salud
Date Issued
24 de marzo de 2020
Type
Article
Volume
37
Issue
1
Series/Report No.
Revista Peruana de Medicina Experimental y Salud Pública
Start Page
51
End Page
6
ISSN
1726-4634
1726-4642
Abstract
OBJECTIVES: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. MATE RIALS AND METHODS: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). RESULTS: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). CONCLUSION: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.
Keywords