Cargando...
Increased Blood Ethanol Elimination in Rats Treated with Halothane
Author(s)
Hospital San Borja Arriarán
María Eugenia Pino
Hospital San Borja Arriarán
T Pereda
Hospital San Borja Arriarán
H Iturriaga
Date Issued
28 de mayo de 2008
Type
Article
Volume
9
Issue
5
Series/Report No.
Pharmacology
Start Page
275
End Page
280
ISSN
0031-7012
1423-0313
Abstract
The effect of chronic halothane inhalation and blood ethanol elimination was studied in the rat. Hepatic α-glycerophosphate, oxidase ADH and malic enzyme activities were determined. Malic enzyme activity was also assayed in adipose tissue. Halothane-treated rats showed an increased ethanol elimination (378 ± 13 vs. 292 ± 19; p < 0.01). Hepatic and adipose tissue malic enzyme activities were significantly enhanced in halothane-treated rats (6.26 ± 1.2 vs. 1.03 ± 0.23; p < 0.002 for the liver and 1.84 ± 0.71 vs. 0.75 ± 0.33 for adipose tissue). Mitochondrial α-glycerophosphate oxidase activity was also significantly increased in the liver of halothane-treated animals (p < 0.05) while no changes in ADH activity were observed. The enhanced ethanol elimination after halothane treatment, a substance which inhibits microsomal enzyme, is possibly due to increased NADH reoxidation. Another possibility explaining augmented ethanol metabolism may be an increased incorporation of ethanol into lipids related to the enhancement of malic enzyme activity.
Keywords