Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS.

Montoro, P; Carbone, V; Quiroz, J de Dioz Zuniga; De Simone, F; Pizza, C

Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS.

Author(s)

Carbone, V

Quiroz, J de Dioz Zuniga

De Simone, F

Pizza, C

Date Issued

2004

Type

journal article

Volume

15

Issue

1

ISSN

0958-0344

Abstract

The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS.