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Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023
Author(s)
Caballero, Francesca
Universidad Peruana Cayetano Heredia
Martinez-Ventura, Anne
Universidad Peruana Cayetano Heredia
Cuicapuza Arteaga, Diego
Universidad Peruana Cayetano Heredia
Fajardo-Loyola, Alex
Instituto Nacional de Salud
Gutierrez-Ajalcriña, Rosmery
Soto-Pastrana, Javier
Asmat-Marrufo, Percy
Barco-Yaipen de Vera, Evelyn
Meza-Fernandez, Henry
Chambi-Quispe, Mario
Pino-Dueñas, Jimena
Laura-Rivas, Nicomedes
Briones-Alejo, Alexander
Diaz-Rengifo, Pilar
Ministry of Health
Peralta-Siesquen, Carlos
Salvatierra, Guillermo
Universidad Peruana Cayetano Heredia
Tsukayama Cisneros, Pablo
Universidad Peruana Cayetano Heredia
Date Issued
19 de junio de 2025
Type
Article
Volume
42
Issue
2
Series/Report No.
Revista Peruana de Medicina Experimental y Salud Pública
Start Page
156
End Page
65
ISSN
1726-4634
1726-4642
Abstract
BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments. OBJECTIVE.: To genetically characterize clinical isolates of uropathogenic Escherichia coli (UPEC) from hospitals in Peru and contextualize them against 127 additional UPEC genomes reported in six Latin American countries between 2018 and 2023. MATERIALS AND METHODS.: The genomes of 16 Peruvian UPEC isolates were sequenced, assembled and supplemented with 127 genomes available in the NCBI public database. Serotypes, sequence types (STs), antimicrobial resistance (AMR) genes, and resistance-associated mutations were identified. A phylogenetic analysis was also conducted in order to determine evolutionary relations and distribution in phylogroups. RESULTS.: The ST131 clone was the most prevalent (42.7%), followed by ST1193 (13.3%). Phylogroup B2 was widely predominant (83.2%), with serotype O25:H4 standing out. The resistance genes blaTEM-1, blaCTX-M-15, and blaCTX-M-27 were identified with high frequency, as well as mutations in gyrA and parC associated with fluoroquinolone resistance, especially in the ST131 clone. CONCLUSION.: Our findings show high circulation of high-risk UPEC clones, such as ST131 and ST1193, in Latin America, along with a notable burden of genes and mutations linked to multidrug resistance, highlighting the need to strengthen regional genomic surveillance. BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments.