Arévalo Ipanaqué, Janet Mercedes

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Arévalo Ipanaqué, Janet Mercedes
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    LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis
    (2026-02-13)
    Eva Dueñas  
    ;
    Ingrid Tirado
    ;
    Percy Huaihua
    ;
    Ariana Parra del Riego
    ;
    Luis Cabrera-Sosa
    BACKGROUND: Tegumentary leishmaniasis is a parasitic disease endemic in the Americas. Its clinical management and control rely on early and accurate diagnosis and adequate treatment. PCR-based molecular diagnostics offer high sensitivity and specificity over microscopy or culture but are less accessible in low-resource settings. New molecular tools for detecting Leishmania infections are needed in rural endemic regions. A promising tool harnessing CRISPR-Cas technology enables highly specific and sensitive detection of nucleic acid targets, offering an exciting potential for portable molecular diagnostics. Previously, we developed CRISPR-Cas12a-based assays coupled to PCR preamplification for Leishmania detection. Here, we adapted our assays, which target the multicopy 18S rDNA and kinetoplast DNA (kDNA) minicircles, by replacing PCR with loop-mediated isothermal amplification (LAMP). METHODOLOGY/PRINCIPAL FINDINGS: LAMP-coupled CRISPR assays were optimized for fluorescence-based and lateral flow readouts. The assays could detect as low as 0.2 genome equivalents per reaction using L. braziliensis M2904 strain genomic DNA. The kDNA assay reliably detected all tested species of the L. (Viannia) subgenus, while the 18S assay showed pan-Leishmania detection capability. There was no cross-reactivity with other protozoan (Trypanosoma cruzi and Plasmodium falciparum) and bacterial (Mycobacterium tuberculosis) pathogen DNA, nor with human DNA. When applied to 90 clinical samples (skin lesions) from the Cusco region of Peru and compared to kDNA real-time PCR, LAMP-CRISPR assays with a fluorescence readout achieved a sensitivity of 90.9% (95% CI: 80.1-97.0%) for kDNA and 72.7% (95% CI: 59.0-83.9%) for 18S rDNA, both with 100% (95% CI: 90-100%) specificity. Overall, lateral flow strip results agreed with fluorescence-based detection in 18 tested samples, with one discrepancy observed in the 18S assay associated with low parasite load. CONCLUSIONS/SIGNIFICANCE: These new proof-of-concept LAMP-CRISPR assays, combining high sensitivity, multiple Leishmania species detection capability, and a portable lateral flow readout, hold promise as next-generation molecular tools to improve leishmaniasis diagnosis and surveillance, supporting One Health strategies for disease control.
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    Ecological divergence and hybridization of Neotropical Leishmania parasites
    (2020-09-21)
    Frederik Van den Broeck  
    ;
    Nicholas J. Savill
    ;
    Hideo Imamura
    ;
    Mandy Sanders
    ;
    Ilse Maes
    Significance Parasites are interesting models for studying speciation processes because they have a high potential for specialization, thanks to the intimate ecological association with their hosts and vectors. Yet little is known about the circumstances under which new parasite lineages emerge. Here we studied the genome diversity of parasites of the Leishmania braziliensis species complex that inhabit both Amazonian and Andean biotas in Peru. We identify three major parasite lineages that occupy particular ecological niches and show that these emerged during forestation changes over the past 150,000 y. We furthermore discovered that meiotic recombination between Amazonian and Andean lineages resulted in full-genome hybrids presenting mixed mitochondrial genomes, providing insights into the genetic consequences of hybridization in parasitic protozoa.
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    Cuidado interprofissional de mulheres indígenas no ciclo gravídico-puerperal à luz da Teoria da Complexidade
    (2026-01-01)
    Julieli Rosso
    ;
    LUANA PIZARRO MENEGHELLO
    ;
    Silvia Naujorks
    ;
    Janet Mercedes Arévalo-Ipanaqué  
    ;
    Dirce Stein Backes
    RESUMO Objetivo: Refletir, à luz da teoria da complexidade, as dinâmicas do cuidado interprofissional à saúde das mulheres indígenas no ciclo gravídico-puerperal. Método: Estudo teórico-reflexivo, empregando o referencial da complexidade de Edgar Morin, por meio do tetragrama para desvelar os encontros, tensões e possibilidades presentes no cuidado interprofissional em contextos interculturais. Resultados: O cuidado interprofissional emerge como estratégia crucial para superar a fragmentação do modelo biomédico hegemônico. A aplicação do tetragrama demonstrou que: a ordem reside nas práticas tradicionais e protocolos biomédicos; a desordem manifesta-se nas rupturas e conflitos culturais; a interação ocorre nas experiências de articulação entre saberes; e a organização indica a construção de arranjos de cuidado mais inclusivos e adaptativos. Conclusão: A teoria da complexidade oferece uma lente fecunda para repensar e (re)orientar a atenção à saúde das mulheres indígenas, destacando a imperatividade da escuta ativa, valorização dos saberes tradicionais e construção de práticas interprofissionais culturalmente seguras, contextualizadas e transformadoras.
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    Multi-country analysis of nursing care management during the COVID-19 pandemic
    (Editorial Ciencias Medicas, 2020)
    Arévalo Ipanaqué, Janet Mercedes  
    ;
    da Silva Tetzlaff, Alessandra Andréa
    ;
    Tiscar-González, Verónica
    ;
    Paola Sanchez, Laura
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    Lobos Rodríguez, Roberto Alfredo
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    Eficacia de un programa de terapia nutricional domiciliaria ambulatoria
    (2025-05-07)
    Huayanay Escandon, Rosamell
    ;
    Valdez Narbasta, Angelina Del Pilar
    ;
    Arévalo Ipanaqué, Janet Mercedes  
    Introduction: Outpatient home nutritional therapy is a care model that allows improving the use of health resources and providing continuity to in-hospital interventions in malnourished patients with intestinal failure. Objective: To evaluate the effectiveness quality -quantitative in patients who are part of an outpatient home nutritional therapy program. Material and methods: Mixed study. The quantitative phase collected the impact indicators of all patients (34), at the beginning of the program and currently. In the qualitative phase, 9 participants were interviewed for theoretical saturation. The quantitative analysis applied descriptive statistics, Anova and Student's T; the qualitative developed transcription, ordering, coding-categorization, description and interpretation. Results: The average nutritional and biochemical indicators increased since the beginning of the program: BMI from 19.95 to 21.88, albumin from 3.63 mg/dl to 3.80 mg/dl, proteins from 7.16 mg/dl to 7.29 mg/dl and lymphocytes from 26.62µl to 29.89µl. Patients and family members presented difficulties that were overcome; They point out strengths of the PTNDA program and perceived satisfaction. Conclusion: BMI is the only indicator that presented significant differences between patients according to years of experience in the program (2 and 3 years). Overall, BMI and lymphocytes have significant differences from the beginning of the program to the present. Patients and family members report a positive impact and satisfaction with the PTNDA for the maintenance and/or improvement of their nutritional status.
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    Professional Quality of Life and Job Stress among Nurses in COVID-19 Areas
    (2024-01-01)
    López Mamani, Enoc Tito
    ;
    Arévalo Ipanaqué, Janet Mercedes  
    ;
    Meneses la Riva, Monica Elisa  
    ;
    Morales Garcia, Wilter Charming  
    ;
    Cabanillas Chavez, Maria Teresa  
    Background: the COVID-19 pandemic has posed unprecedented challenges in the healthcare field, notably affecting nursing professionals working in critical areas. These challenges have impacted their professional quality of life (PQoL) and increased job stress levels, which are crucial for both the well-being of healthcare personnel and the quality of care provided to patients. Objective: this study aims to explore the relationship between professional quality of life and job stress among nurses at a medium-complexity hospital in Lima, Peru, during the COVID-19 pandemic. Methods: a quantitative, non-experimental, cross-sectional, correlational methodology was used, with a non-probabilistic sample of 59 nurses. Data were collected through online questionnaires assessing PQoL and job stress, using validated tools such as the Nursing Stress Scale and the PQoL-35 Questionnaire. Results: the majority of participants (69,5 %) perceive their PQoL as good, highlighting high levels of intrinsic motivation and perceived quality of life. In contrast, workload was the most negatively valued aspect. Job stress was perceived as low overall, and a moderate positive correlation between PQoL and job stress (r = 0,517; p < 0,05) was found, suggesting that better working conditions could improve PQoL. Workload was identified as a significant factor of job stress. Conclusions: despite the challenges imposed by the pandemic, nurses maintain a positive perception of their PQoL, especially in terms of intrinsic motivation and perceived quality of life. However, workload is highlighted as a critical factor of job stressBackground: the COVID-19 pandemic has posed unprecedented challenges in the healthcare field, notably affecting nursing professionals working in critical areas. These challenges have impacted their professional quality of life (PQoL) and increased job stress levels, which are crucial for both the well-being of healthcare personnel and the quality of care provided to patients. Objective: this study aims to explore the relationship between professional quality of life and job stress among nurses at a medium-complexity hospital in Lima, Peru, during the COVID-19 pandemic. Methods: a quantitative, non-experimental, cross-sectional, correlational methodology was used, with a non-probabilistic sample of 59 nurses. Data were collected through online questionnaires assessing PQoL and job stress, using validated tools such as the Nursing Stress Scale and the PQoL-35 Questionnaire. Results: the majority of participants (69,5 %) perceive their PQoL as good, highlighting high levels of intrinsic motivation and perceived quality of life. In contrast, workload was the most negatively valued aspect. Job stress was perceived as low overall, and a moderate positive correlation between PQoL and job stress (r = 0,517; p < 0,05) was found, suggesting that better working conditions could improve PQoL. Workload was identified as a significant factor of job stress. Conclusions: despite the challenges imposed by the pandemic, nurses maintain a positive perception of their PQoL, especially in terms of intrinsic motivation and perceived quality of life. However, workload is highlighted as a critical factor of job stress
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    Anxiety, stress and depression in emergency nurses covid-19
    (Fundacion Index, 2020)
    Obando-Zegarra, Roxana
    ;
    Arévalo Ipanaqué, Janet Mercedes  
    ;
    Aliaga Sánchez, Ruth América
    ;
    Obando Zegarra, Martina
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